Once the reaction between DTSSP and proteins or peptides is complete, the disulfide-containing crosslinked protein can be cleaved via reduction of the crosslinkers disulfide bond using a reducing agent, such as 2-mercaptoethanol (CovaChem 11301-10x1) or DTT (CovaChem 11302-10x5). CovaChems technical support team is readily available to assist with any individual optimization.

Dithiobis(sulfosuccinimidyl propionate) (DTSSP) is the water soluble version of Lomants Reagent (DSP). Both DSP and DTSSP have been used extensively in protein crosslinker applications as well as other scientific applications involving forming a stable linkages between the disulfide group and metal surfaces, while leaving the NHS functional groups available to react with other nucleophiles. This application can be useful for immobilizing proteins or other molecules to the surface of gold beads or other foil.

Due to the lipophobic nature of the sulfonate groups on DTSSP, this crosslinker does not permeate the cell membrane. This feature makes DTSSP particularly useful for crosslinking membrane proteins on the surface of the cell without interfering with intercellular proteins.

Package Sizes: 100 mg and 1 gram

Product Numbers: 13304-100 and 13304-1

Product Name: DTSSP Crosslinker

Alternative Names: DTSSP; 3,3-Dithiobis(sulfosuccinimidyl propionate); Dithiobis(sulfosuccinimidyl propionate); 81069-02-5; cleavable Sulfo-NHS crosslinker; DTSSP Protein Crosslinker

Specifications

Appearance: White Solid

Purity: is ≥ 90% Storage: 4 °C, protected from moisture

Properties

Properties Physical State: Solid

CAS#: 81069-02-5

Chemical Formula: C₁₄H₁₄N₂Na₂O₁₄S₄

Molecular Weight: 608.51

Spacer length: 12.0 Å

DTSSP Product Information BulletinDTSSP Crosslinker MSDS