DTSSPisacleavableproteincrosslinkingreagentthatcontainsadisulfidemoeityinitseightatomspacerarm.DTSSPisamembraneimpermeable,homobifunctionalcrosslinkerthatisreactivetowardsamine(amino)functionalgroups.InthepHrangeof7-9,thisreactionisparticularlyselectiveforprimaryaminegroups,resultingintheformationofstableamidelinkages.PhosphatebuffersandphosphatebufferedsalinearetypicallyusedforcrosslinkingreactionsinvolvingDTSSP,althoughothersnon-aminecontainingbuffersmayalsobeused.

OncethereactionbetweenDTSSPandproteinsorpeptidesiscomplete,thedisulfide-containingcrosslinkedproteincanbecleavedviareductionofthecrosslinkersdisulfidebondusingareducingagent,suchas2-mercaptoethanol(Covachem11301-10x1)orDTT(CovaChem11302-10x5).CovaChem"stechnicalsupportteamisreADIlyavailabletoassistwithanyindividualoptimization.

Dithiobis(sulfosuccinimidylpropionate)(DTSSP)isthewatersolubleversionofLomant"sReagent(DSP).BothDSPandDTSSPhavebeenusedextensivelyinproteincrosslinkerapplicationsaswellasotherscientificapplicationsinvolvingformingastablelinkagesbetweenthedisulfidegroupandmetalsurfaces,whileleavingtheNHSfunctionalgroupsavailabletoreactwithothernucleophiles.Thisapplicationcanbeusefulforimmobilizingproteinsorothermoleculestothesurfaceofgoldbeadsorotherfoil.

DuetothelipophobicnatureofthesulfonategroupsonDTSSP,thiscrosslinkerdoesnotpermeatethecellmembrane.ThisfeaturemakesDTSSPparticularlyusefulforcrosslinkingmembraneproteinsonthesurfaceofthecellwithoutinterferingwithintercellularproteins.

PackageSizes:100mgand1gram

ProductNumbers:13304-100and13304-1

ProductName:DTSSPCrosslinker

AlternativeNames:DTSSP;3,3"-Dithiobis(sulfosuccinimidylpropionate);Dithiobis(sulfosuccinimidylpropionate);81069-02-5;cleavableSulfo-NHScrosslinker;DTSSPProteinCrosslinker

Specifications

Appearance:WhiteSolid

Purity:is≥90%Storage:4°C,protectedfrommoisture

Properties

PropertiesPhysicalState:Solid

CAS#:81069-02-5

ChemicalFormula:C₁₄H₁₄N₂Na₂O₁₄S₄

MolecularWeight:608.51

Spacerlength:12.0Å

AdditionalProductInformation:

DTSSPProductInformationBulletin
DTSSPCrosslinkerMSDS

CovaChem是一家化学生产公司，为生命科学领域的研究科学家服务。我们致力于生产市场上最高质量的试剂，用于质谱分析、蛋白质组学、蛋白质科学、药物测试和药物开发。CovaChem的产品包括用于products for protein research and proteomics, UltraHigh Purity LCMS mobile phases, GC derivatization reagents（蛋白质研究和蛋白质组学的产品、超高纯度LCMS流动相、GC衍生试剂）生命科学研究中使用的其他化学品和定制有机合成服务。 系列产品名称CAS NumbeSizePart No.摘要ZERO LENGTH CROSSLINKERSN,N’-Carbonyldiimidazole (CDI)N、 N’-羰基二咪唑530-62-15 grams13501-5N、 N’-羰基二咪唑用于活化羟基或羧酸基团，以与亲核试剂（如胺或羟基）结合。25 grams13501-25N,N’-Dicyclohexyl carbodiimide (DCC)N、 N’-二环己基碳二亚胺538-75-05 grams13502-5N、 N’-二环己基碳二亚胺（DCC）是合成应用中广泛使用的零长偶联剂。该试剂通常用于制造肽键、NHS酯和其他酯化合物。DCC由于其非极性环己基环，仅用于有机溶剂中。25 grams13502-25N-Ethyl-N’-(3-dimethylaminopropyl)carbodiimide HCl (EDC-HCL)N-乙基-N’（3-二甲氨基丙基）碳化二亚胺盐酸盐25952-53-83 x 10 mg13503-3x10N-乙基-N’（3-二甲氨基丙基）碳化二亚胺盐酸盐（EDC HCl）是一种零长度蛋白质交联剂。也被称为EDAC HCl，该试剂通常与磺基NHS（CovaChem 13505）或NHS（CovaChem 13504）一起使用。EDC通过激活蛋白质或其他分子的羧基区，并将其与胺或其他亲核官能团偶联而起作用。MES-DryBlend（CovaChem 19214）和PBS-DryBlend（CovaChem 19213）预制缓冲包最适合EDC偶联反应。Alternative Name 别名：EDAC-HCL; EDC; 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide HCl100 mg13503-1005 grams13503-525 grams13503-25100 grams13503-100BN-Hydroxysuccinimide | (NHS)N-羟基琥珀酰亚胺6066-82-625 grams13504-25N-羟基琥珀酰亚胺（NHS）用于与EDC（CovaChem 13503）、DCC（CovaChem 13502）和其他偶联剂的偶联反应，以稳定活性中间体或形成稳定的酯供日后使用。N-Hydroxysulfosuccinimide (Sulfo-NHS)N-羟基磺基丁二酰亚胺106627-54-7500 mg13505-500N-羟基磺基丁二酰亚胺（Sulfo-NHS）是EDC反应中常用的催化剂（CovaChem 13503）。Sulfo NHS的作用是EDC中的催化剂促进了偶联，因为它稳定了活性中间体，提高了整体偶联效率和产率。1 gram13505-15 grams13505-5HOMOBIFUNCTIONAL CROSSLINKERS同双功能交联剂包含两个具有相同分子结构和反应专一性的反应官能团。在NHS和磺基NHS酯以及双马来酰亚胺化合物中一些更常见的双官能团。Bis-(Sulfosuccinimidyl) glutarate (BS2G Crosslinker)双磺基丁二酰亚胺戊二酸100 mg13302-100双磺基丁二酰亚胺戊二酸（BS2G）是一种水溶性蛋白质交联剂。BS2G是DSG的水溶性类似物（CovaChem 13301）。BS2G极性磺酸基团防止其渗透到细胞膜中，使其非常适合细胞表面交联应用。Alternative Names别名: Sulfo-DSG; Sulfosuccinimidyl glutarate; Di-(N-sulfosuccinimidyl) glutarate sodium salt;  BS2G-d0